The quartet stayed out until the early hours of Sunday morning despite their involvement in Monday afternoon's reserve clash with Rochdale.
Club rules state players should not be out late 48 hours before a game and those concerned were ordered to train on their own at the Seasiders' Squires Gate training base on Wednesday.
Boss Ian Holloway told the Blackpool Gazette: "I am very proud of my lads. They have been brilliant this season and it is important they understand that. But I am only proud when they behave the right way. When they don't, it lets myself, the team and the fans down.
"Last weekend, some of them were playing on Monday but they were out on the Saturday till God knows what hour in the morning. That is unacceptable, so I have spoken about that to the whole squad.
"For me, Monday's game was absolutely vital. Maybe they thought differently because it wasn't a first-team match. But it is about a game, and how dare you disrespect the game and not prepare properly for it?"