The Spanish club won 4-2 on penalties after a 0-0 draw which provided 120 minutes of scoreless action at the Juventus Stadium in Turin.
The Sevillistas had some strong scoring chances in the first half through Carlos Bacca and Alberto Moreno before Benfica lifted their game only to be denied by Portuguese goalkeeper Beto on numerous occasions.
Bacca sent a well-struck drive past the post early in extra-time before both sets of players settled for a shootout which was eventually dominated by the Spaniards.
Beto was able to deny Oscar Cardozo and Rodrigo from the spot to guide Unai Emery's Sevilla to a 4-2 win which handed them their third UEFA cup, adding to the back-to-back feat of 2006-07.
For Jorge Jesus' Benfica, it was their eighth loss in a European final since their 1962 triumph.
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