The Bhoys host Barcelona at Parkhead in the Champions League and the Nigerian has vowed to again stop the Argentine ace who plans to produce a dummy in honour of his newborn son Thiago if he manages to find the back of the net.
"First of all I will tell Messi congratulations. It's a great thing to become a father but I believe we are going to ruin his party in Glasgow," said Ambrose who produced a shackling performance on Messi in Barcelona's last-gasp 2-1 win at the Nou Camp two weeks ago.
"Yes, Barcelona's tradition is to keep possession of the ball no matter where they are playing.
"But the most important thing is we know how they play. We did well against them in Spain, and we will make them fight for their possession. That's where our strengths lie and we will give everything we have to ensure we don't lose and we get at least a point."