Sunderland skipper Lee Cattermole jarred his knee in a practice match, and aggravated a previous injury which has kept him on the sidelines.
The Black Cats midfielder is now likely to miss the Barclays Premier League trip to Wigan.
Boss Martin O'Neill said: "He had a bit of a setback there. We are certainly hoping it's not as long as it was before, that would be terrible.
"But it is a setback and I would say he would be doubtful for tomorrow."