Messi was unable to play any part in Barcelona's 5-0 league win over Real Mallorca at the weekend but Roura is hopeful the star forward, who scored the opener in last week's 2-2 in Paris, will feature in the return at the Nou Camp.
"We hope he'll be able to play," Roura told a press conference.
"He is reacting well but we will have to wait a while before we know how he feels and what the doctors' tests say."
Roura warned, however, that the club are not prepared to take any risks with Messi, who has scored eight goals in eight starts in the Champions League season.
"I've said before that if a player plays it is because it has been guaranteed that he is capable of playing. We will see what happens, there is still a bit of time left," he said.