Juan Mata struck twice after Valencia teammate Ruben Baraja had his goal cancelled out by Thierry Henry. Samuel Eto'o netted a late consolation for Barca.
"I do not believe that it will affect the team much. The feeling is that we fought very hard and had very good moments in the game. The problem came when conceding their goals, but I am proud of how the players behaved. Perhaps we deserved more and I am with the team.
"The team demonstrated to have will. No-one can say this team has not fought. We failed in things, but it is not possible to say that we did not have the right attitude throughout the encounter."
Asked about comments from Barca midfielder Xavi and sports director Txiki Begiristain that the team lacks "on field maturity", Rijkaard added: "I do not know if we lack maturity, but this team has great experience. We went to 3-1 in a moment and that was crucial in the final result. It hurts."