Atleti captain Gabi told a press conference that the players were fired up to finally get one over their rivals.
"We have 14 years of desperation to win this game," he said.
"Real Madrid are a special rival because of what it means to play against them and the excitement around the fixture.
"It has been a long time since we arrived at a derby so evenly matched and with the opportunity to go level on points with them, and it's been a very long time since we beat them."
The midfielder emphasised the good form his side are in and believes they have what it takes to end their barren run against Madrid.
"It's clear we are in a very good moment, we have just beaten Sevilla away from home and we are focused on the task ahead. On Saturday we have a great opportunity.
"We have spent a long time talking about the best way to win a derby and now we have to go and do it."