Arsenal lose unbeaten record in Seville
Arsenal had not been beaten in a competitive match since the start of April, but goals from Seydou Keita, Luis Fabiano and former Tottenham striker Frederic Kanoute saw victorious Sevilla join the already-qualified Gunners in progressing through to the last 16.
Eduardo Da Silva scored for Arsenal but it was not enough to save Arsene Wenger's men, and they will need to beat Steaua Bucharest in their final match and hope Sevilla slip up against Slavia Prague to clinch first place.
The two teams went into the match at the Ramon Sanchez-Pizjuan with starkly contrasting domestic records this season.
Arsenal had been unbeaten and boast a three-point lead at the top of the Premier League table, while Sevilla had lost seven of their 12 La Liga matches and already find themselves 14 points adrift of pacesetters Real Madrid.