The Reds, who enjoy a 1-2 lead from the first leg, will host the Bundesliga side at Anfield for the second leg on Wednesday night.
Klopp said: "Anfield is a main factor, we all know it, it's a very special place. Nothing happens by itself - we have to perform, we have to deliver - but then we all know the atmosphere can and will help you.
"I really hope that everybody is ready for this game, everybody is ready to fight. I would be concerned if the people thought it was a weak side coming from Hoffenheim and we need to be dominant and things like this and each bad pass gets a moan.
"It will not happen because I know we have a football crowd and they know about the quality and so they are ready to fight. And we are ready to fight too."