"We are starting our journey again now," said Ancelotti who picked up a 0-0 draw in Sardinia before the international break.
"We have recovered some important players and Ronaldinho benefited from the break by regaining some of his form.
"Alessandro Nesta will be on the bench, but he is feeling better and will probably come on during the second half.
He added: "I do not trust any team, especially not one having difficulties, as every game is the right chance for them to prove themselves.
"Sampdoria are a very organised side and have always put in good performances, even if the results didn't go their way."