"The game could prove very significant when it comes to who actually finishes in the Champions League spots and that's why we are going there to win.
"With a victory we would be right back in the European Cup race. But even if we lose, it wouldn't be the end for us.
"[Third-placed] Juventus? They have more points than us and Fiorentina. Juve also have just the League to play for which is a massive advantage for them - even if the season is still long."
Although Ancelotti is hoping for a victory, he is well aware of the threat which the Viola will pose at the Franchi.
"I expect a lively and mature Fiorentina side," he predicted. "The youngsters in their squad are growing and they have Adrian Mutu. He attacks space and is very dangerous."